What can Cryo-EM do for you?
The Cryo-EM facility (RRID:SCR_026324), a MSU core, is a regional facility that brings state-of-the-art cryo-EM capabilities, including single particle analysis and cryo-electron tomography, to hte Northern Rocky Mountains. Instrumentation includes a 200kV Talos Arctica with a Gatan K3 camera, 120kV Tecnai Spirit, and a Vitrobot Mark IV. Collectively, these instruments allow imaging of biological structures at single nanometer to subnanometer resolutions, and thus enables 3D modeling of cells, viruses, proteins, necleic acid, and other soft matter at the atomic level.
Access to the Montana State Cryo-EM Core Facility is available to internal MSU users, external academic users, and external commercial users.
Services
Please view our instrumentation page for more details on available instrumentation.
Single Particle Analysis
Single Particle Analysis (SPA) is capable of achieving high-resolution 3D structures for many proteins or macromolecular complexes.
For single particle analysis, current collection rates are up to 10,000 images/day via SerialEM. The facility offers live, on-the-fly data analysis, and is equipped for full remote operation and can transfer data between institutions via Globus.
Cryo-electron Tomography
Cryo-electron tomography (CET) is a revolutionary technique that allows for detailed data collection of complex biological samples, illuminating previously hard to interrogate phenomena such as virus-cell interactions, or localization of sub-cellular structures.
Acknowledgements
Acknowledgement of core facility services and instruments supported by Montana State University are required in publications. Learn more about how to acknowledge Cryo-EM in your publication here.